Drug Loading in Protein-Based Nanocage Through Covalent Attachment

Covalent attachment is a common strategy of drug loading during conjugation of drug to protein nanocages. Amino acid side residues (either natural or genetically engineered) on the nanocage, such as cysteine, lysine, tyrosine and glutamic and aspartic acid have evidently been used in such conjugations. Now, Creative BioMart Nanocage can offer you with professional rotein-based nanocage through covalent attachment service according to your scientific needs, if you have any needs in this regard, please feel free to contact us.

Figure 1. CPMV-DOX targets and kills cells efficiently. (] Aljabali A, et al. 2013)

Drug Loading in Protein-Based Nanocage Through Covalent Attachment We Offer

• We provide genetic modification, which can generate new modification sites by generating unique reactive cysteine residues in protein nanocages.
• In most cases, many molecules have been encapsulated in the internal cavity or attached to the outside of the protein nanocage through their endogenous amino acids. We can use the lysine or cysteine residues on the outer surface of the protein nanocage to study the covalent binding of dox to the protein nanocage through amide bonds or disulfide bonds.
• Moreover, we can incorporate unnatural amino acids, such as homopropynylglycine (HPG) and azidohomoalanine (AHA), to attach drugs of the desired density at specific locations.
• In addition, our covalent drug binding service allows controlled release. For example, disulfide bonds and hydrazine bonds broken under weakly acidic conditions can mediate drug release in a reducing environment.

Our Advantages

• Our drug loading in protein-based nanocage through covalent attachment service can avoid or reduce the structural changes caused by the environment of the nanocage.
• Our drug loading in protein-based nanocage through covalent attachment service can attach the drug with the required density at a specific location, and it can also mediate the release of the drug.
• Creative BioMart Nanocage will complete your project on time and efficiently. We have professional after-sales service: which has been established to provide customers with efficient, fast, practical and feasible solutions.
• We have established a professional after-sales service team to provide customers with efficient, fast and practical solutions.

Service Procedure

Please inform us of your protein nanocage drug loading details through online consultation. We will reply to you in time and provide you with a comprehensive evaluation report on your project.

Why choose us?

Creative BioMart Nanocage provides professional protein nanocage drug loading to meet the scientific research needs of customers. Creative BioMart Nanocage relies on world-class technical expertise, we provide customers with the best quality one-stop protein nanocage drug loading, including the development of experimental procedures according to different experimental needs. Please feel free to contact us for more detailed information, our scientists will tailor the most reasonable plan for your project.

References

• Lee E J, Na K L, Kim I S . Bioengineered protein-based nanocage for drug delivery. Advanced drug delivery reviews, 2016, 106(Pt A).
• Aljabali A , Shukla S, Lomonossoff G P , et al. CPMV-DOX delivers. Molecular Pharmaceutics, 2013, 10(1).
• Stephanopoulos N, Tong G J, Hsiao S C, et al. Dual-surface modified virus capsids for targeted delivery of photodynamic agents to cancer cells. Acs Nano, 2010, 4(10):6014-20.