Protein Nanocage Mass Spectrometry

Mass spectrometry (MS) is a powerful and highly accurate technique to identify proteins and analyze protein primary sequence. In the application of ferritin and virus-like particles (VLPs) as drug delivery platforms and vaccines, correct molecular weight and a 100 % sequence accuracy of the protein nanocage are indispensable for the achievement of the theoretical conformation and bioactivity. In many cases, mutations or fusions with foreign peptides of protein nanocages are employed to incorporate desirable functions with protein nanocages. The verification of these intended alterations is necessitated before any further characterizations. When using eukaryotic expression hosts to express protein nanocages, the post-translational modification sites and types need to be identified and their impact on protein nanocage function needs to be assessed. In addition, during the preparation of protein nanocages, unexpected degradations of protein nanocages are likely to occur and cause problems in the sequential characterizations and evaluations. Mass spectrometry is a direct and highly sensitive tool to shed light on the mechanism behind these degradations.

Creative BioMart Nanocage has long-term experience in mass spectrometry of protein nanocages. Our goal is to offer fast, reliable, and affordable MS services to meet our client's expectations. We provide a variety of sample pretreatments including enzyme digestion, protein concentration, protein desalting, and purification through gel electrophoresis to reduce sample preparation time for our clients. MALDI-TOF MS and LC-MS/MS services are available for different research purposes.

Table 1. Mass spectrometry services

Mass Spectrometry Service	Description
MALDI-TOF MS	MALDI-TOF MS primarily produces singly charged ions from proteins with reduced fragmentation compared with other ionization methods. It is well-suited for the analysis of biomacromolecules such as proteins. Applications: subunit molecular weight determination for protein nanocages and post-translational modification analysis.
LC-MS/MS	We use the bottom-up LC-MS/MS strategy to obtain detailed and accurate primary structure information of protein nanocages. Applications: subunit sequencing, ratio determination of various types of subunits, post-translational modification detection, and degradation detection.

Service Advantages

Affordable. Sample pretreatments are free of charge. No hidden charges.
Fast and high quality. Well-trained MS experts and advanced devices.
Flexible. We accept various types of protein nanocage samples. MS service can be customized to meet the special needs of clients.

If you are interested in our MS service, please do not hesitate to contact us through online inquiry.

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Project Description:

Creative BioMart Nanocage has extensive expertise and experience in nanocages research for drug delivery, medical imaging development. We have established a comprehensive and complex platform for nanocages fabrication and characterization. Our mission is to provide reliable and high-quality services to provide strong support to our customers in the design, preparation, characterization, modification, and evaluation of nanocages.