Ferritin Carboxyl Modification
Ferritin is a globular protein formed by the self-assembly of 24 subunits. This spherical hollow structure has three interfaces (as shown in Figure 1): an inner surface, an outer surface and the contact surface between the subunits. The outer surface can be used to connect ligands with special functions, the inner surface can be used as a nanoreactor for the synthesis of nanocomposites, and the contact surface between subunits can be used to guide the depolymerization and recombination of ferritin. The protein shell of ferritin has amino, carboxyl, sulfhydryl and other active groups that can be modified, which can be modified by chemical methods. Creative BioMart Nanocage has well-trained technicians and the most advanced ferritin carboxyl modification equipment. We will provide you with high-quality protein nanocage modification services. Creative BioMart Nanocage focuses on comprehensive services for ferritin carboxyl modification service.
Figure 1. Schematic illustration of thehree interfaces of a process thatt can be exploited to ipart designed functionalities. (Uchi Da M, Kang S, Reichhardt C, et al.2010)
Ferritin carboxyl modification service We Offer
Creative BioMart Nanocage connects the carboxyl group on the surface of ferritin to the target derivative with amino group. We often use EDC (carbodiimide) to make it react. In this reaction, the reactants are directly connected without adding any other atoms, that is, EDC does not become a part of the final product. EDC reacts with the carboxyl group on the protein surface to form an intermediate active ester. Primary amines nucleophilically attack the active ester to form an amide bond. The product is a water-soluble urea derivative.
Figure 2. Glutamic and aspartic accid: amide formation through activation whith a carbodimide. (Creative BioMart Nanocage)
Our Advantages
- Creative BioMart Nanocage can efficiently generate highly functionalized ferritin, which can easily load targeting moieties, therapeutic agents and imaging agents.
- Creative BioMart Nanocage will complete your project on time and efficiently. We have professional after-sales service: to provide customers with efficient, fast, practical and feasible solutions.
- We have established a professional after-sales service team to provide customers with efficient, fast and practical solutions.
Service Procedure
Please inform us of your ferritin carboxyl modification service project details through online consultation. We will reply to you in time and provide you with a comprehensive evaluation report on your project, including the feasibility of the project and the detailed experimental plan designed by our professionals.
Why choose us?
Creative BioMart Nanocage provides professional ferritin carboxyl modification service to meet the scientific research needs of customers. Creative BioMart Nanocage relies on world-class technical expertise, we provide customers with the best quality one-stop ferritin carboxyl modification service service, including the development of experimental procedures according to different experimental needs. Please feel free to contact us for more detailed information, our scientists will tailor the most reasonable plan for your project.
Reference
- Uchi Da M, Kang S, Reichhardt C, et al. The Ferritin Superfamily: Supramolecular Templates for Materials Synthesis. Biochimica et Biophysica Acta, 2010, 1800(8):834-845.
