Heparosan

Introduction to Heparosan

Many therapeutics have issues with delivery because of the nonoptimal pharmacokinetics or detrimental side effects due to their nonhuman nature. Therefore, the pharmaceutical industry has employed a variety of delivery strategies such as utilizing a newly introduced system using a natural material. Heparosan is a naturally occurring sugar polymer produced by the body that is stable and inert in the blood stream while being biodegradable. It is a member of the glycosaminoglycan (GAG) family which has similar structure with hyaluronan.

Heparosan is superior in drug delivery due to its perfect biocompatibility and stability. Our body produces no extracellular enzymes that degrade heparosan and there are no known receptors or binding proteins for heparosan. These qualities allow heparosan for long circulation time in the blood.

Fig1. A novel redox-sensitive heparosan-cystamine-vitamin E succinate (KSV) micelle system was constructed for intracellular delivery of doxorubicin (DOX). The preparation and antitumor effect of redox-responsive DOX/KSV micelles. (Lipeng Qiu, 2020)

Service Details

With many years of experience in the field of polymer science and proteins, Creative BioMart Nanocage has developed different novel heparin modification methods. For example, our narrow size distributed heparosan that can offer improved pharmacokinetics and drug performance properties. We will provide you with professional heparosan production, characterization, and modification services. Heparosan synthesis usually involves modern isolation and chromatography techniques such as microbial fermentation extraction, metabolically engineered bacteria production, and recombinant enzyme synthesis in vitro.

• Microbial fermentation extraction method: This is a method of producing Heparosan using microorganisms. By selecting suitable microbial strains, such as E. coli, they are genetically engineered to produce Heparosan. After the fermentation process, the target product is obtained through a series of extraction and purification steps.
• Metabolic engineering engineered bacteria production method: This method works by engineering the metabolic pathways of the microbe so that it can efficiently synthesize Heparosan. This usually involves editing the microbial genome to enhance or build specific metabolic pathways that increase Heparosan production.
• Recombinant enzyme synthesis in vitro: This method uses enzymes expressed in vitro to catalyze the synthesis of Heparosan. These enzymes can be naturally occurring or modified through genetic engineering techniques to improve their catalytic efficiency and specificity.
• Purification process: After the synthesis of Heparosan, a series of purification steps are required to increase its purity. This may include the use of multiple separation methods, such as precipitation, filtration, centrifugation, chromatography, etc., to remove impurities and obtain a high purity Heparosan.
• Chromatographic technology: During the synthesis of Heparosan, chromatographic techniques can be used to further purify and refine the product.

Why choose us?

• The expert team of Creative BioMart Nanocage has a multidisciplinary background, including medicine, pharmacy, molecular biology, bioinformatics, etc. We attach great importance to innovation and practice.
• We work with scientists from many pharmaceutical and biotech companies. We pay special attention to heparosan-based delivery related services and are committed to contributing to more customers.
• We can provide customized services. Not limited to the types of services mentioned above, products can be designed according to your specific needs.
• We will complete your project on time according to your scientific research needs. We have a strict quality management system to provide customers with efficient, fast and reliable solutions.
• We keep pace with The Times, focus on market demand, and constantly innovate technology.

Creative BioMart Nanocage provides heparosan-based delivery design services to meet the scientific research needs of customers. Please feel free to contact us for more detailed information, our scientists will tailor the most reasonable plan for your project.

References

1. Qiu, L.; et al. Redox-responsive biocompatible nanocarriers based on novel heparosan polysaccharides for intracellular anticancer drug delivery. Asian J Pharm Sci. 2020;15(1):83-94.
2. Zha, Z.; et al. Heparosan-based self-assembled nanocarrier for zinc(II) phthalocyanine for use in photodynamic cancer therapy. Int J Biol Macromol. 2022;219:31-43.