High-performance Liquid Chromatography
Background
In high-performance liquid chromatography (HPLC), a mixture sample is being pumped at a high pressure to pass through a column (stationary phase) and its components flow out from the column at different time points to be analyzed by the connected detectors. Its mechanism resembles that of fast protein liquid chromatography (FPLC), but it has a higher separation resolution because of the higher pressure and the smaller resin particles of the columns. According to the purpose of HPLC, it can be divided to be analytical HPLC and preparative HPLC. They differ in column volume, column particle size, HPLC device pump capacity, and HPLC device tubing size. Analytical columns typically have particle sizes of 3-5 µm whilst preparative column has particle sizes of over 10 µm.
Fig 1. High-performance Liquid Chromatography
Creative BioMart Nanocage offers both analytical and preparative HPLC services. We employ analytical HPLC to determine protein nanocage purity, quantify protein naocage, and analyze protein nanocage properties. Preparative HPLC is well-suited to purify challenging protein nanocage in which FPLC fails due to a relatively low resolution.
Analytical HPLC Service
Creative BioMart Nanocage possesses a wide selection of HPLC columns for different analytical purposes. Detectors of our analytical HPLC devices include UV/vis detector, photodiode array detector, fluorescence detector, refractive index (RI) detector, conductivity detector, multi-angel laser light scattering (MALS) detector, and mass spectrometer.
Table 1. Analytical HPLC service
| Type | Columns and usages |
|---|---|
| High-performance size-exclusion chromatography (HPSEC) | We have high-performance size-exclusion columns for characterizing protein nanocages with different molecular weights. For example, the TSKgel UP-SW, SW, SWXL, and SuperSW series from TOSOH. They are suitable to detect protein nanocage hydrodynamic volume and molecular weight. |
| High-performance ion exchange chromatography (HPIEC) | We own strong and weak ion exchange columns made by TOSOH. They can be used to determine the purity of protein nanocages. |
| High-performance reverse-phase liquid chromatography (RP-HPLC) | C4, C8, and C18 columns are available. They are well-suited for purity determination and quantification. If coupled with a mass spectrometer, RP-HPLC can be used to determine protein molecular weight and analyze protein primary sequence. |
Preparative HPLC Service
We have a full range of preparative HPLC columns along with preparative HPLC devices to conveniently and reproducibly scale up our client's analytical HPLC. Our preparative HPLC service covers ion exchange, reverse-phase, hydrophobic interaction, and size-exclusion chromatography.
Why Choose Us
- Comprehensive service. We offer both analytical and preparative HPLC services, covering all main types of HPLC.
- Experienced staff and advanced facilities. With the advanced HPLC devices and the wide selection of HPLC columns, our HPLC experts can help our clients solve protein nanocage characterization and purification problems rapidly and effectively.
If you are interested in our analytical and/or preparative HPLC service, please do not hesitate to contact us through online inquiry.
