E2 Protein Nanocage Service

The E2 protein is derived from the E2 core domain (dihydrolipoamide acetyltransferase) of the pyruvate dehydrogenase multienzyme complex. The protein is formed by 24 subunits in E. coli or 60 subunits in Geobacillus stearothermophilus that self-assemble into a hollow structure with a cubic core or an icosahedron with twelve 5-nm openings (PDB ID 1B5S), respectively. Pyruvate dehydrogenase enzyme complex is composed of three enzymes: pyruvate decarboxylase (E1), dihydrolipoamide acetyltransferase (E2) and dihydrolipoamide dehydrogenase (E3) (Figure 1). It has been shown that the complex can be produced separately and the component enzymes can be reassembled in vitro to form the native structure.19 Because E1 and E3 do not dissociate from each other in the presence of the enzyme E2, it is evident that the latter forms the structural core of the assembled complex.

Figure 1. (a) E1-E2–E3 complex of Geobacillus stearothermophilus active site coupling model. (b) Electron micrograph of wild-type E2 protein. (Bhaskar S, Lim S.2019)

E2 Protein Nanocage Service We Offer

The modification of the external surface with functional ligands does not hinder the assembly of the E2 core domain. This property is identical to that in its natural state, in which the E2 domain is linked to two distinct folding protein domains at its surface. Similar to other natural protein nanocages, we can modify E2 protein nanocages in different ways to achieve multiple functions, such as drug loading and attachment of specific targeted proteins. We offer you with the following modification service:

• E2 Protein Nanocage Chemical Modification
• E2 Protein Nanocage Genetic Modification
• E2 Protein Nanocage Internal  Modification
• E2 Protein Nanocage External Modification
• E2 Protein Nanocage Subunit–Subunit Interfaces Modification

Our Advantages

• Creative BioMart Nanocage provides you with a variety of E2 protein nanocage service for your choice, our modified protein nanocages can easily loaded with targeting moieties, therapeutics, and imaging agents.
• Creative BioMart Nanocage will complete your project on time and efficiently. We have professional after-sales service: which has been established to provide customers with efficient, fast, practical and feasible solutions.
• We have established a professional after-sales service team to provide customers with efficient, fast and practical solutions.

Service Procedure

Please inform us of your E2 Protein Nanocage Service project details through online consultation. We will reply to you in time and provide you with a comprehensive evaluation report on your project, including the feasibility of the project and the detailed experimental plan designed by our professionals. As our condition optimization is completed, the result report and final product will be delivered to you immediately.

Why choose us?

Creative BioMart Nanocage provides professional E2 protein nanocage serviceto meet the scientific research needs of customers. Creative BioMart Nanocage relies on world-class technical expertise, we provide customers with the best quality one-stop E2 protein nanocage service, including the development of experimental procedures according to different experimental needs. Please feel free to contact us for more detailed information, our scientists will tailor the most reasonable plan for your project.

Reference

•  Bhaskar S, Lim S. Engineering protein nanocages as carriers for biomedical applications. Npg Asia Materials, 2017, 9(4):e371.