Protein Nanocage Purification
Creative BioMart Nanocage concentrates on the purification of protein nanocages, including ferritin, engineered ferritins, virus-like particles (VLPs), and chimeric VLPs. Purification is the process of removing impurities while maintaining the target protein conformation and bioactivity. It is crucial to quality control. The impurities may cause side effects and toxicity in the application of protein nanocages as a vaccine, drug delivery platform, or imaging reagent. The correct structure and high final purity are essential to ensure protein performance in the downstream characterization and evaluation. A key principle in purification is to make use of the biochemical property differences between target protein and impurities. Charge, size, hydrophobicity, and affinity to ligands are common properties laying the foundation of purification techniques. As each target protein differs in biochemical property and impurity composition, the purification pathway is specific to the target protein to a certain degree. Our purification experts are highly experienced in various purification techniques such as protein precipitation, protein chromatography, ultracentrifugation, membrane separation, and endotoxin removal. We also provide gel electrophoresis and high-performance liquid chromatography services to characterize the purity of protein nanocages and as alternatives for purification.
Protein Precipitation
Our protein precipitation service covers a variety of methods, inclusive of thermal precipitation, ammonium sulfate precipitation, PEG precipitation, PEI precipitation, and isoelectric precipitation. We adopt orthogonal experiments for condition screening and use statistical tools to find out the optimal condition setting. Protein precipitation is an ideal approach as an initial step for protein nanocage purification.
Protein Chromatography
Our service includes ion exchange, hydrophobic interaction, size exclusion, and multimodal chromatography. Chromatography adopting 96-well plate for high throughput screening, small lab-scale column, and large pre-industrial scale column are available for selection.
Membrane Separation
Creative BioMart Nanocage provides microfiltration, ultrafiltration, and membrane chromatography for protein nanocage purification. Traditional pressure-driven ultrafiltration and advanced techniques such as electro-ultrafiltration, ultrafiltration under ultrasonic field, and high-performance tangential flow ultrafiltration are offered. Membrane chromatography mainly covers ion-exchange and hydrophobic interaction types. Different purification scales are available in our membrane separation service.
Ultracentrifugation
We provide both preparative and analytical ultracentrifugation services. Preparative ultracentrifugation includes differential ultracentrifugation and density-gradient ultracentrifugation. It is suitable for standard protein nanocage sample preparation. Analytical ultracentifugation such as sedimentation velocity (SV) and sedimentation equilibrium (SE) experiment reveals information about size, shape, mass, association state, and aggregation state of protein nanocages.
Endotoxin Testing and Removing
In the preparation of recombinant protein nanocages, especially those expressed by E. coli, endotoxin contaminant is common. Creative BioMart Nanocage offers limulus amebocyte lysate (LAL) test and rabbit pyrogen test to detect endotoxin activity of customer's sample. We utilize various endotoxin removal methods to help customers strictly control the sample endotoxin level to meet the requirements for further research.
Gel Electrophoresis
We offer native-PAGE, SDS-PAGE, and agarose gel electrophoresis services to characterize protein nanocage and determine the purifty.
High-performance Liquid Chromatography
We employ analytical HPLC to determine protein nanocage purity, quantify protein naocage, and analyze protein nanocage properties. Preparative HPLC is adopted to purify challenging protein nanocage.
Our Advantages
- A correct protein nanocage conformation after purification is guaranteed.
- Different levels of final protein purity and final product mass are available to customers for selection based on the research aims and needs.
- Fast turnaround.
- Strong technician support.
- No charge if purification fails.
If you are interested in our protein nanocage purification service, please do not hesitate to contact us through online inquiry.
